BDNF released from CGB scaffold promoted neuronal differentiation of NSCs and led to significant differences in differentiation rate and average neuron perimeter compared with the control group. The present invention relates to a layered chitosan scaffold wherein said layered scaffold comprises at least two fused layers, wherein at least one of the. The CGB scaffolds released BDNF in a uniform profile, whereas the CB scaffolds only released BDNF during the first 3 days. We aimed to construct a biodegradable transparent scaffold for culturing corneal endothelial cells by incorporating chitosan nanoparticles (CSNPs) into chitosan/polycaprolactone (PCL) membranes. There were on average more cells on the CGB scaffold on the first day than on any other day sampled (P < 0.05). There were no significant differences in diameter and porosity of individual CGB scaffolds (P > 0.05). Chitosan-based 3D-printed scaffolds for bone tissue engineering - ScienceDirect International Journal of Biological Macromolecules Volume 183, 31 July 2021, Pages 1925-1938 Review Chitosan-based 3D-printed scaffolds for bone tissue engineering L. Finally, we investigated whether the released BDNF can induce NSC to differentiate into neurons. The amount of BDNF released from CGB over a 30 day period was determined by ELISA. Prior to this, the diameter and porosity of CGB scaffolds were measured. The viability of hUC-MSCs adhered to the CGB scaffold was determined by digesting with 0.25% trypsin and evaluating with the cell counting kit-8 (CCK-8). Fluorescent nuclear staining (Hoechst 33342) was employed to determine the attachment of the hUC-MSCs to CGB scaffolds on the 1st, 3rd, 7th and 10th day of co-culture. hUC-MSCs were co-cultured with the CGB scaffold. At the same time, the scaffold prepared by the sol-gel transformation of SiO 2 nanoparticles-PVA/CS mixed solution had good cyto-compatibility, which could significantly. The chitosan scaffold based on immobilization of BDNF by genipin (GP) as a crosslinking agent referred to hereafter as a CGB scaffold was prepared by freezing-drying technique. In addition, N-guanidinium-chitosan acetate/silica hybrid scaffolds containing either sulfonate or carboxylate groups could function as template to induce biomineralization. This study tested the cytotoxicity of a BDNF blended chitosan scaffold with human umbilical cord mesenchymal stem cells (hUC-MSCs), and the in vitro effect of BDNF blended chitosan scaffolds on neural stem cell differentiation with the aim of contributing alternative methods in tissue engineering for the treatment of traumatic brain injury (TBI).
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